FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 n_120_3.3410000000d9bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 n_120_3.3410000000d9bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487555
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGAGACCGCCCATGGCTGCAGCTGCGGTTCCAGCTGCAGGTGCAACCC15220.31216990903590364No Hit
GCCGGAAGAGCACCGCACGTCGCGCGGTGTCCGGTGCGCCCCCGGCGGCCC7070.14500928100419438No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.12572940488765372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG552.744855E-936.8153324
AGACCGC2100.036.4257476
ATACTTA458.6915315E-734.99728814
GACCGCC2300.033.2582937
TCGATAC1150.033.25829337
CCGCGTA352.814206E-432.15025720
TAGCCGG501.9797226E-631.49755913
GAGACCG2500.031.4975595
TAATAAG801.0004442E-1030.93827642
CGATACC1250.030.59762838
ATAAGTA751.6989361E-930.0007544
CGCGATA453.4886103E-529.99767534
CGTAAAC300.005146334429.99767510
ACCGCCC2600.029.4207958
CACGCTA702.8812792E-828.92632926
CAACCTA1100.028.63414628
GCGGGTT803.3542165E-928.1488041
TATGCCG803.3887773E-928.12282237
CGAAGGT406.1638025E-428.12282214
TGCGGTT2800.028.1228226
Produced by FastQC (version 0.11.2)