FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 n_120_2.3410000000d985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 n_120_2.3410000000d985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575509
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTTCAAGGTCACCGTGCAGCCGTACGCCGCCGGCGCCGGCAGGTTCG11210.19478409547027065No Hit
CCGGGGTGGAGCACGTCGGCGGCGACATGTTCGCCTCCGTGCCCCGCGGCG9540.16576630426283515No Hit
GCGGCGGCTACGGAGGAGGTGGCGGCGGCGGCTACGGGCGCCGTGAGGGCG8820.15325563979016837No Hit
GCCGTTGCGGCGCAGCGCCCCGAGGGGCGCGCCAGAGGCGCGCGGTGACCG7510.13049318081906625No Hit
CTGAAGCGTGGGTACGTGGCCTCCAACTCCAAGGATGACCCTGCCAAGGAG7460.12962438467513107No Hit
GGGAGGGAAGCGGATGGGGGCCGGCGATGCGCCCCGGCCGTATGCGGAACG6700.11641868328731611No Hit
CGCTCATGCAGAAGCTCCGCGCCGTGCTCGTCGAGCACGCCCTCACCAGCG6590.11450733177065867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATT800.039.371582
GTTAGAG301.1392136E-437.49999615
CGTTTAG552.741217E-936.8213845
CTACGGA1150.035.2143338
AACGATT458.695806E-734.996962
GGACTAT653.6743586E-1034.61237735
AGCCGTA2350.034.4710822
CGTCTCA1450.034.1349643
CCGTTTA606.4155756E-933.75293444
GTGGGTA1950.033.458638
AAGCGTG2100.033.2114034
GGGTAAT950.033.2040561
CCGTATG1350.031.66391638
CTTACCG501.979346E-631.50000242
GAAGCGT2000.031.4972653
ATTTGCG1000.031.4972656
AGGGCTA651.39971235E-831.15655543
GGTACGT2100.031.0687311
TATGCGG1400.030.5330641
ATGACCC2150.030.346235