FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 n_120_1.3410000000d96b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 n_120_1.3410000000d96b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535619
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT14950.2791163121547219No Hit
GCTCCTTGTGCCTCAGCGCGAGCTTGTCGATCGCCTTCTTGATCACCTCGT9500.17736488063343533No Hit
GCCTCCTCCTCCTTCTTCTCCATCGCCGGCGAGAGAGAGAGAGAGTTAGCT7720.1441323029989601No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.13666430802492072No Hit
GCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAGGGATTT6930.1293830129252323No Hit
GGAAGGGGCTCGCCGTTCCACTCCTTGAGGCAGTCGAGCATGGGGTCGATG6480.12098151857943801No Hit
GTCGCACATCACAAGGATGTTGTCGCCCCTCCTGAACGGGTCCTTGAAAAT5650.10548542900830629No Hit
CTAAGCTCTCCACCATTGCTTGCAAAGCTGGTCGTGTCACTCCTCCCGTTA5530.10324503051609446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC2200.039.90101621
CCGTTAA2200.039.90101620
CCCGTTA950.037.8980445
GCGATTT2450.037.677451
TAACGAA2350.037.33670824
GTTAACG2450.035.82948322
TCGCACG702.1827873E-1135.3536227
CGATTTG2650.034.8078542
GAGTTAG1100.034.7725143
TCCGTTA2550.034.42440419
AATTCCG2550.034.42119216
GAACGAG2550.034.40833728
GCGTATT1000.033.771861
ATTCCGT2600.033.76239817
CGAACGA2600.033.7466427
GCGGGTA950.033.17007419
ACGAGAC2700.032.49676530
ACGAACG2700.032.49676526
CGTTAAG1050.032.13965639
TTAAGTC1050.032.1396567
Produced by FastQC (version 0.11.2)