FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 k_60_3.3410000000d7a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 k_60_3.3410000000d7a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407072
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTACTTATTCCGTGAGTCGGAAGCGGGGCCTGGCCCCTCCTTTTGGCTCT7900.1940688625108089No Hit
GTCAGTATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCT6170.15157023818882165No Hit
GGGAAGTTGAGCTTGGCCCTGTCCCCGCGGATGGCGCGCGCGGCGTCGTCG5530.13584820375756623No Hit
GCCATTTCTTGTCGATGAGGTCCTCCGGCGCCGCGCGCTCCAGCACGCCGT5480.13461991981762442No Hit
GGAGACCTTCTCTGAGTACCCTCCTCTTGGTCGTTTTGCCGTGCGTGACAT4670.11472171999056678No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.10931727065482273No Hit
GGGAGATGGAGCAGGCCGACCCCGCCGCGTCCCCGCGCTGCGGCGCGCGCT4100.10071928307522993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC1050.042.8558545
TATCGCT1150.039.129266
GACTAAC356.2396357E-638.5702722
AGTATCG1250.035.998924
CCGCGGA3000.035.99891725
TGAGCCG1000.035.9989178
TTCGCCC1400.033.74898541
ATCGCTT1650.032.7262887
TATTCCG1050.032.141897
GGAAGTT4100.030.7307832
ATTCCGT1100.030.6808998
GTGAGTC1100.030.68089913
CGCGCAA453.4860786E-529.999125
TCGCTAG453.4860786E-529.999131
CGCACGA300.005144153329.999139
TACGTGC300.005144153329.99919
CCCTTAT751.6971171E-929.99909832
GGGAAGT4450.029.8709281
CGCTTCG1900.029.6043749
TGAGTCG1150.029.34694514
Produced by FastQC (version 0.11.2)