FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 k_60_2.3410000000d72a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 k_60_2.3410000000d72a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656151
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGATTGCTCTGATCACCACCACGTGGTAGATGGCCGCCAGTGCCGCTCC8090.12329479037599578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT253.8881528E-545.001622
GTTTACG504.8752554E-835.9985354
TTACGTC504.8752554E-835.9985356
CCCTTAG300.00514477230.00106817
GCGCTTT453.4895522E-529.99878136
TTTACGT554.1626736E-628.6351995
ACCGATT654.8600305E-727.69751544
ACGTTTA903.947207E-1027.49888211
CGTTTAT903.947207E-1027.49888212
TATTCGC507.144648E-527.00507545
CATACTC1550.026.1279723
CGTTAGT450.001226284124.99898527
AGTGCCG1450.024.82657841
CACGTTT1001.3424142E-924.74899510
TGACACG551.367706E-424.54445627
GCGGACT1201.6370905E-1124.37586819
TGCGTTA651.5229352E-524.2297862
ACCGTTC751.696395E-623.99902515
CGGTCAG851.8904211E-723.82437721
GCATGAC1450.023.27491627
Produced by FastQC (version 0.11.2)