FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 k_60_1.3410000000d695.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 k_60_1.3410000000d695.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925531
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT801.0004442E-1030.93534535
TATAACG856.4828782E-926.46874639
GGTCCCG901.1912562E-824.9982597
TAACGAC952.1158485E-823.68256241
GCCTATA1052.3665052E-923.56978836
GTCGCTT1304.9112714E-1122.5154631
CCCTAGG702.7037131E-522.49843238
CTACGGA1003.645073E-822.49843212
TAACCGC855.0264316E-621.17842944
CACGTCA1700.021.17499714
CCTAGGC754.6038578E-520.99853939
TAGCGTC550.00393755520.4531219
TCGACTC2000.020.24858931
GTCGGTT1009.0570575E-720.24858937
GTCGACT1800.019.99860630
CCCACGT1703.6379788E-1219.85155912
CCTATAA1151.5956721E-719.56385637
GCCCCTA706.985337E-419.2843736
CACGCTA706.985337E-419.2843714
ACTATAC1656.002665E-1119.08957914
Produced by FastQC (version 0.11.2)