FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 k_120_3.3410000000d886.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 k_120_3.3410000000d886.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560182
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGATCGAGGCCGCCCGCGTCGCCGTCGCCAACGGCACCGCCCCCGTCG20600.36773762812800126No Hit
CCTGAAGATACCTACATTCTCGAGGCCGCTGAAACTGCCGGGGTGGAGCTG9260.16530341924588793No Hit
GTTTTGTTTCCTCGCTGCTCAGCATTGTGATTCTTAACCTGCCCCCCGCTG8920.1592339632476588No Hit
CCCTTCTCCACACTCTTTGCTCGCAGAAGGTTCATCCCCTGCGCGTAGCTG7640.1363842465484432No Hit
GCCGGGTTGGCGGTTGGTTCCGCGGTTTGCATTACGTCCATCTTCGCAGGG6870.12263871384657128No Hit
GGGAAGTAGAACACAATGCTGTAAAGCCAGATAACGCCAGCCCAGCCCCAG6760.12067506631773246No Hit
CCCAACTCCTCTCCTCTCCTTCCCACTACTCTCGCGAGGAGGAAAGCGAAG6130.10942872137983727No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGG6020.10746507385099842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT2500.037.80097643
CGCGCTA552.741217E-936.8224244
GCGCTAG606.4119376E-933.75388345
CCCGCGT2900.033.3629316
CATTACG950.033.15579230
ACGGCAC3050.032.45695534
TAACGCC708.1308826E-1032.14081632
GTATGCG751.6989361E-930.00077425
TAGCGGG602.4146902E-730.00077216
AACGATC901.2732926E-1129.9980989
TTACGTC1050.029.99809632
TCTTAAC1500.029.99809532
CCCGAGT751.7007551E-929.99809537
AACGGCA3300.029.31632233
TAACGCT702.8823706E-828.9267338
ACTCTAA1250.028.7981729
CGCGTAG1250.028.79817242
CCAACGG3400.027.79235531
GTCACGT654.8685797E-727.690556
CCCACTA1150.027.39201222