FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 k_120_2.3410000000d843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 k_120_2.3410000000d843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838597
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCATTGGCCCTGGTGTCTATGACATCCACTCCCCAAGGATCCCGTCCA19690.23479692867968763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGT2800.033.7485242
GTCTATG3150.030.00405518
CTATGAC3200.029.53524220
GTGTCTA3200.029.53524216
CTGGCAT4100.028.0317121
TCCCGTC3500.026.99881743
TATGACA3550.026.62331621
TGTCTAT3700.026.15218417
ACTCCCC3650.025.88773531
GGATCCC3750.025.79733340
TGGTCCG1058.54925E-1125.7116289
GATCCCG4000.025.30988541
GAGTCGC1251.8189894E-1225.19739512
CCCGTCC3900.024.23548944
CTCCCCA3900.024.2297132
ATCCACT4100.023.59512127
TTACCCG1052.3665052E-923.5689938
AGTCGCG1353.6379788E-1223.33092113
CCCTGGT4050.023.33092111
TCTATGA4100.023.05189519
Produced by FastQC (version 0.11.2)