FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 k_120_1.3410000000d80f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 k_120_1.3410000000d80f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821215
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGGAGGAGAAGGT9530.11604756367090226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA450.001225908325.00121942
TTGCGAC450.00122677924.99817332
TCGACTG2250.024.99817330
TTTCGAC551.3684163E-424.54366332
GTCCCGT1101.5097612E-1024.54366314
TATTGGT1600.023.90450528
AGTATCG1052.3665052E-923.56970626
CCCCATA1800.022.50383619
CGTTCAT2500.022.4983563
GGGCGTA1003.6439815E-822.49835612
CGACTGC2500.022.49835631
CGTCAAT1305.0931703E-1122.49835624
CCGTTAT500.002265025222.4983563
TCGAAAG903.2868775E-722.4983565
ACCGTAA602.4678977E-422.4983548
TATCGAA1204.5110937E-1022.49835428
GTTGACT1257.530616E-1021.5984216
CGTGAGT1056.08361E-821.43483745
CCATATA1700.021.1800821
TTCCGTC1301.2241799E-920.77277221
Produced by FastQC (version 0.11.2)