FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_input_5_pchx_pn.3410000000d8f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_input_5_pchx_pn.3410000000d8f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319952
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCAAAACACTAACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTG33981.062034305145772No Hit
CCTCCATGCGCACCTTGAAGCGCATGAACTCCTTGATGACGTCCTCGGAGG24540.7669900485072761No Hit
GCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGT23620.738235735360304No Hit
GTTTGCCCGACGACAATGAAGCGAATTTGCAGGCAACACGGAATCTCGCGG22860.7144821723258489No Hit
AGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGG21730.6791643746561984No Hit
TTGCCCATTTCACTGCTGCAATCACTTGACGCCCACCTAACATGTTGAGTG20950.6547857178576787No Hit
TGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCATCTTCAACGA19900.6219682952442867No Hit
CCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGG19820.6194679201880282No Hit
CTGGGCTTTCTCCGACGGTGGAGGAAATGGTTTTCACCACGCAACCTCCGG18630.5822748412261839No Hit
CCATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGG17110.5347677151572736No Hit
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG17040.5325798869830475No Hit
CCATGAGAGGTTGGAGTCCCAGCGCTCTCATCCACCGTTCGTGATCGCTTG16640.5200780117017553No Hit
AGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGT16360.5113266990048507No Hit
CCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAG15490.4841351202680402No Hit
TTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAG15220.47569635445316794No Hit
ACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAG14600.4563184477671651No Hit
AGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGG14490.45288043206480977No Hit
GCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG14450.4516302445366805No Hit
TGAACGATTCACTTTCTTGATCTTCCTCGATGGCCACCGCGAGATTCCGTG14380.4494424163624544No Hit
TGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCT14240.44506676001400214No Hit
TGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCG14110.44100365054758217No Hit
CTCAACCACCTCATGCATGGAGTCCAGAAGCTTTGTCAGTTGGTAAAACCG13510.4222508376256438No Hit
TCTCGCTCAGACTTGGGTTCCATGTCAGCATGGGAGCGTTCTTGCCAATGG13320.41631244686703No Hit
TCTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACATCCGCTCGGTGG13150.4109991498724808No Hit
TCCCACGCTTTTTCTCTGTTTTCTTCTTCTCCTTAACCACACCGTTAACAG13100.40943641546231935No Hit
CCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCCCTGCCGACAG12820.40068510276541475No Hit
CTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGC12540.3919337900685103No Hit
AGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAG12510.3909961494224134No Hit
TGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATC12370.3866204930739611No Hit
GCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGG12260.38318247737160577No Hit
CCAGCTTTGACGGTAGCTGCATGGGTCAAATCTGCATGTCTACAACCGACA12170.38036955543331497No Hit
TGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGG11810.369117867680152No Hit
TACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACG11460.35817872680902135No Hit
TAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAG11260.35192778916837525No Hit
CCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGG10890.34036355453317996No Hit
TGAAGCTGAAGGACGGCGGCCACTACGACGCCGAGGTCAAGACCACCTACA10650.3328624293644047No Hit
CATGCGACGTCGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCAC10070.314734710206531No Hit
GGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCC9860.3081712256838526No Hit
GGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGG9490.2966069910486573No Hit
AAGAATTCTCGCGGCTTGATCACGCCTTGCACTACAACGTGCGTGGTACAC9440.29504425663849576No Hit
TCTCTGCAGAAACATGAGCCACCATTGAGAAAAGCTCGTCCAGCGACTCCC9260.28941841276191427No Hit
CTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAG9110.2847302095314297No Hit
ACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACG8940.2794169125368805No Hit
TTCCTCAGCCTGTGGTTCAGTCTTCTGATCCAGTAAATGAGAAAATCAACG8740.27316597489623445No Hit
CAGTCTTCACGGCGAGTTCTGTTAGATCCTCGATCTGAATTTTTGACTCCA8670.2709781467220083No Hit
TTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAG8620.2694154123118468No Hit
GACTCGTTTCAGCTTTGTGATTGAACGATTCACTTTCTTGATCTTCCTCGA8530.26660249037355604No Hit
TGTAACTGTCGTCGCCCGTGTAAGAGCTCTGGAGAGATATTGCAAAACAAG8350.26097664649697455No Hit
AGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAG8160.25503825573836075No Hit
GGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGG8150.25472570885632845No Hit
CTCTGTTCCAGATTCAGCATGGAGAATTATGACCACACTCAACATGTTAGG8070.25222533380007No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGG7940.24816222433365004No Hit
GCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCG7660.2394109116367455No Hit
GATCGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAG7250.226596489473421No Hit
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG6200.19377906686002902No Hit
TGAACAATGATAATAATAGCAACAACAACTTATACGCTCCCCCAAAGGAAG5670.17721408211231685No Hit
ACACCATCTCCAGAAAGGCCAGGGAGTCGCTGGACGAGCTTTTCTCAATGG5590.1747137070560584No Hit
TCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGT4910.15346051907786168No Hit
GTTTGACCGTGTGTCCAAGGGATGGAACTAACGGCCATGGAAGTCAGGTCG4740.1481472220833125No Hit
ACTCCAAAACACTAACCAACCTTCTTCTAGCTTCTCAAAGCTTTCATGGTG4490.14033355003250486No Hit
TCCACCAGTCATGCTAGAATCGATAGATCTGCTAGAGTCAGCTTGTCAGCG4490.14033355003250486No Hit
AAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTG4400.13752062809421414No Hit
TCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAG4340.13564534680202028No Hit
GTGATCCGGAGGTGGAGACCCAGCTTTCTTGTACAAAGTGGTGATATCCCG3610.11282942441366206No Hit
ACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCGTTCATCACCCT3440.10751612741911286No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC3200.10001500225033755TruSeq Adapter, Index 15 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTA950.045.0154816
GCGCTAC207.013751E-445.0154818
GGTATTA1150.044.99437713
ACGGCCC2000.043.8900916
TAGCGAA1750.043.72248544
AGCGAAG1750.043.72248545
AATACGC1550.043.54294224
AAGCGCA2850.043.43598618
ACGGGAG1350.043.32791534
CGTGTAA1000.042.76470617
ATGCGCA3000.042.7446566
CCATGCG2950.042.7065284
CCGCTTA1950.042.6869749
ATAGCGA1800.042.50797343
AAGCGGA1650.042.267448
CCGACAG3100.042.1046745
TGCGCAC3000.041.994757
CAGCGCT2100.041.80008720
CGGCCCC2100.041.80008717
TACGATA1400.041.7804928
Produced by FastQC (version 0.11.2)