FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_input_3hrs_pchx_pn.3410000000d992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_input_3hrs_pchx_pn.3410000000d992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609943
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTG52990.8687697047101123No Hit
CCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTG40260.6600616778944918No Hit
GCCTTTGCCCATTTCACTGCTGCAATCACTTGACGCCCACCTAACATGTTG29810.4887341931951018No Hit
CACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCA26400.4328273297668798No Hit
CTTTGCCCATTTCACTGCTGCAATCACTTGACGCCCACCTAACATGTTGAG25340.41544865667775516No Hit
TTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCG24080.39479098866615403No Hit
CCGGAGGTTGCGTGGTGAAAACCATTTCCTCCACCGTCGGAGAAAGCCCAG23770.3897085465363157No Hit
CCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCG23430.38413425516810584No Hit
CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACC21870.3585580947727902No Hit
GCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCC21660.35511515010419004No Hit
AAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTGTGAAGAT21170.34708161254412295No Hit
TGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGAC20460.33544118056933186No Hit
CGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACGGGGCCGTCGG20300.3328179846313508No Hit
ACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGG19400.31806250748020715No Hit
TGCTTCTCTGTTGTGTTCTCAGCTGCTGATGGAGAAGAGTAAGGGAGGAAG17890.29330609581551065No Hit
TCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCG17530.2874039049550532No Hit
CCACCATTGGCAAGAACGCTCCCATGCTGACATGGAACCCAAGTCTGAGCG17180.28166566384071956No Hit
TCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTG16880.276747171457005No Hit
AGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTC16830.27592742272638593No Hit
TGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCCCTGCCGAC16830.27592742272638593No Hit
CTTCCCACGCTTTTTCTCTGTTTTCTTCTTCTCCTTAACCACACCGTTAAC15570.25526975471478486No Hit
CTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGG14910.24444907147061282No Hit
GCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGT14810.24280957400937467No Hit
TGGATGTTTCTCATGGCATTTGCCCTGGGTTGGAGATCATACAGACAATCA14660.2403503278175174No Hit
AATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTG13750.22543090092024992No Hit
ACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCA13710.22477510193575465No Hit
AAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCG13470.22084030802878302No Hit
GCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTG13460.22067635828265922No Hit
GCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGG13160.21575786589894466No Hit
TCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAG12230.2005105395094296No Hit
CCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAAT12140.19903499179431522No Hit
ACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTG12120.19870709230206757No Hit
AATCCAGTGACCTGCAGGCATGCGACGTCGGGCCCAATTCGCCCTATAGTG11790.19329675067998156No Hit
CCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTG11220.18395161515092395No Hit
GTGACGTAGAGCTCGGACTCCCGGGTCGAGTTTTCCGTCAGAAATTGCCTG11100.18198421819743812No Hit
CCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACG10950.17952497200558085No Hit
TCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGG10200.16722874104629448No Hit
CTGGGGCTTTAGAGATGCCTGTCTTGAACACCATCTCCAGAAAGGCCAGGG9800.16067075120134178No Hit
TGATGAGCCTAGTCCCACCAGAGAACACAGACAACTACTGTGTGATCAAAG9250.15165351516453177No Hit
TATTCCAGCAAAGAATTCTCGCGGCTTGATCACGCCTTGCACTACAACGTG9090.1490303192265507No Hit
AAGGAACACCGGAGACAGAGCTGATCTTCTCATCATCGCCACCGGAGGTTG8630.14148863090485503No Hit
GCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGA8380.13738988725175957No Hit
TCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGAT7980.13083189740680687No Hit
AAGATCTAAGTCGTCAACATCCATTAGAAGCTCCCTTGACCTCGAAGGTTG7710.1264052542614638No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC7500.1229623095928636TruSeq Adapter, Index 15 (98% over 51bp)
CTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATG7220.11837171670139668No Hit
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG6750.11066607863357723No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC6460.10591153599598652TruSeq Adapter, Index 3 (97% over 37bp)
AACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAG6140.10066514412002434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCCG2600.042.401846
ACGGGGC2600.041.536539
CCCAGCC2650.040.7527913
CGCGCGT1900.040.261255
GCACACG4150.040.11858411
CCCCGTA2750.040.089018
CCCACGC2200.039.8844764
CGGGGCC2800.039.37313540
GGGCCGT2750.039.27408642
GCGTTAT2200.038.8617979
GCCCCGT2850.038.6823777
CGCGTGA2100.038.569626
CATTACG2750.038.45272435
CCCCGCG2000.038.2481882
CGTCTGA4300.038.19899416
CGTTAAC2300.038.15349245
TCCCACG2300.038.1503643
CGTAATG2950.038.1337911
CCCCTGA2200.037.84221616
CACACGT4400.037.83911512
Produced by FastQC (version 0.11.2)