FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_input_30_pchx_pn.3410000000d95f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_input_30_pchx_pn.3410000000d95f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416831
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC188064.511660601058942TruSeq Adapter, Index 16 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC93812.250552382140484TruSeq Adapter, Index 15 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC88812.1305996914816796TruSeq Adapter, Index 14 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC86412.0730223999654536TruSeq Adapter, Index 13 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC83722.008487852391017TruSeq Adapter, Index 18 (100% over 51bp)
GCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGC33730.8092008511842929No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC31940.766257787928441TruSeq Adapter, Index 3 (97% over 37bp)
CGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTC26190.6283121936708163No Hit
TGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCC25580.6136779654104421No Hit
CTCCTTGATGACGTCCTCGGAGGAGGCCATCGGATCCTTATCGTCATCGTC24540.5887278057534109No Hit
CAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCAC20590.49396518013295554No Hit
GTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGAC19010.45606012988477346No Hit
CAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCCA18350.4402263747178113No Hit
GGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTGGGTGCCCTC17830.42775129488929564No Hit
CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGC15820.3795303132444564No Hit
CTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCC14680.35218109977424905No Hit
GCTGATCTTCAAGAATGTCTCGAGATCCCTAGATCCTCCCGCACGAAAATC11940.28644702529322436No Hit
GTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTC11050.2650954463559572No Hit
CATTTCCCCGAAAAGTGCCACCTGATGCGGTGTGAAATACCGCACAGATGC10890.2612569602548755No Hit
TGCTCTCGCAGGAGCTCCCTAGATTTGTCGTTACATCATGAACTAAGAGCC9640.23126878759017444No Hit
CATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACA9440.22647067996382228No Hit
TTACCGTCACATGAGCCATGAGAGGTTGGAGTCCCAGCGCTCTCATCCACC8940.21447541089794184No Hit
TCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAAC8020.1924041158167219No Hit
TCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCC7970.19120458891013384No Hit
AACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTC7000.16793376692232584No Hit
TGATCGCTTGTGACCATTGCTTGGTGGTAACTCAGGCGAACCATTTGGCTC6220.1492211471795524No Hit
TAGCCTCTTCAATTTCAGAAAGAATGCTGACCCACAGATGGTTAGAGAGGC5530.1326676758686374No Hit
TCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGC5370.12882918976755567No Hit
GAGCTCCCTAGATTTGTCGTTACATCATGAACTAAGAGCCTTACGATTTTC4360.10459874625447724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC61350.044.81495345
CGTATGC61500.044.59589444
TCTCGTA61800.044.48863241
TCGTATG61750.044.48821643
CTCGTAT61700.044.4513442
ATCTCGT62300.044.1315840
CATCTCG39350.043.9691339
GCACACG63100.043.9286311
ACGTCTG63300.043.8964715
CGTCTGA63250.043.89559616
ACACGTC63200.043.89472213
ACCCGTC19650.043.8533232
CTGAACT63200.043.82352419
AGTCACC20200.043.77311728
ACTCCAG63050.043.7493623
CGTCCGC8850.043.7271833
CACACGT63400.043.72076812
CCGTCCA19750.043.63128334
CCCGTCC19750.043.63128333
GTCTGAA63700.043.6208217
Produced by FastQC (version 0.11.2)