FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_input_10_pchx_pn.3410000000d928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_input_10_pchx_pn.3410000000d928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330400
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGG27610.8356537530266344No Hit
CCCTTACTCTTCTCCATCAGCAGCTGAGAACACAACAGAGAAGCATAACAG25290.7654358353510896No Hit
GGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCC23860.7221549636803875No Hit
GCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAG22930.6940072639225182No Hit
AACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCG18460.5587167070217918No Hit
CGTCTTTGTAACTTGCTTTGATTGTTACCGTTCTCATTTCTGAGCCTGATG17690.535411622276029No Hit
CTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCC17230.5214891041162227No Hit
CGGTGGCGATGATGAGAAGATCAGCTCTGTCTCCGGTGTTCCTTCTTTCCG15530.4700363196125908No Hit
TGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCG14820.44854721549636806No Hit
CGAGAATCTCAGCAAGCGCGTTTTGGCGACTCTCATTGCATATCTGTGTTG13170.3986077481840194No Hit
ACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGG12520.3789346246973366No Hit
TTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCG12310.37257869249394676No Hit
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG10700.3238498789346247No Hit
CCAGAAAGGCCAGGGAGTCGCTGGACGAGCTTTTCTCAATGGTGGCTCATG10650.3223365617433414No Hit
CTCTCCTCAGTTCCTAAGGAAGGTCTGAAGAGCCAAGAGTTATTTGATGAG10600.32082324455205813No Hit
CACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGA9190.27814769975786924No Hit
ACAGGAGCCCAGACCTGAGCCAAAACGTGTTGTTCGGTTGATTCTTTGAAG8210.24848668280871672No Hit
GAGCTCGGTACCGGATCCAATTCCCGATCGTTCAAACATTTGGCAATAAAG8170.24727602905569007No Hit
AACGCAGTACCCACTAGTCCATTATGCGCTCATGTTCAAGTTGACCACTTG7810.23638014527845036No Hit
AGAAACTTACACCTGGATGACCAAATGACCCTGCTACAGTACTCATGGATG7800.2360774818401937No Hit
TCTTCTCCATCAGCAGCTGAGAACACAACAGAGAAGCATAACAGTTTCCAG7680.2324455205811138No Hit
CTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGTTGGTTAGTG7520.22760290556900725No Hit
CCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAGACGAGAG7420.2245762711864407No Hit
TCAACATCCATTAGAAGCTCCCTTGACCTCGAAGGTTGAGTAGTGACGCCG7080.2142857142857143No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC6830.20671912832929784TruSeq Adapter, Index 15 (98% over 51bp)
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT6700.20278450363196127No Hit
ATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGGTGGCACT6550.19824455205811137No Hit
AAGGAGCCCGGGTGATCCACCAACCGTGAAGAGCGGATCTTGATTTGGGAC6070.18371670702179177No Hit
GCCGCCTGCAGGTCGACTAGAATAGTAAATTGTAATGTTGTTTGTTGTTTG6050.18311138014527845No Hit
AACTAACGGCCATGGAAGTCAGGTCGAGGCCTCCATCAGTACCTTGAACAG5610.16979418886198547No Hit
TGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACA4900.1483050847457627No Hit
GTACTGTAGCAGGGTCATTTGGTCATCCAGGTGTAAGTTTCTGAAGCCTGG4850.14679176755447942No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC4840.14648910411622276TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC4740.14346246973365617TruSeq Adapter, Index 13 (98% over 51bp)
ACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGG4400.13317191283292978No Hit
AGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGT4370.1322639225181598No Hit
TTATGGGTCTCACATACAACTGTCAGCACTTCGAGAATCTCAGCAAGCGCG4330.13105326876513318No Hit
TGCTACTCGAAATCTTTGCCACCCGTCTCGTTCCAGTTCCTAGCCGCAAGG4280.12953995157384987No Hit
GCAGGAGCTCCCTAGATTTGTCGTTACATCATGAACTAAGAGCCTTACGAT4080.1234866828087167No Hit
TAACGTAACTCGAGAGCCAAATCTTCTCCGATCTAAGACCCGCGTTCTCCG3930.11894673123486682No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC3930.11894673123486682TruSeq Adapter, Index 18 (100% over 51bp)
TTCCTCGAACCCGATAGATTTTCATCGCCCATTCATCGTCCTGGTCGAGGG3920.11864406779661016No Hit
CGAGATGTCCCCATGGGCATCGATTCCACCCGCCTAGGTTGGATGGGCGTG3840.1162227602905569No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC3720.112590799031477TruSeq Adapter, Index 14 (98% over 51bp)
TCGGACATCCGGCCGTCCTGGTCGACGAATCCTCGGACCCGGTCGATGAAG3710.11228813559322035No Hit
CTCGTCCTGGTCGACAAACCCTCGACCACTCCTCGGACACGGTCGATGAAG3680.11138014527845036No Hit
CTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT3460.10472154963680387No Hit
CGGGTTTACTCACCCGTTGACTCGCACACATGTCAGACTCCTTGGTCCGTG3360.1016949152542373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGC2350.044.9974338
GTTACCC1550.043.55248617
TAGCGCC2450.043.16079739
AATAGCG2900.042.66997513
CCTAGCG2500.042.29758537
CACTCGT2400.042.185095
CCCCGCG2650.041.6013959
GCCCGCT2550.041.4744943
CGCCCGC2550.041.46821642
GAGGCCC3000.041.25388722
ATAGCGA3000.041.24764314
GGCCCGC3000.041.24764324
GCGTTAC1650.040.90675415
TGGCACG2700.040.83140
GAGCACA4200.040.7119569
GCGAAGA3050.040.57759517
AGCGAAG3050.040.57759516
GATCGCC3050.040.57145334
GCACCCA2500.040.4976912
TGCACCC2500.040.4976911
Produced by FastQC (version 0.11.2)