FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_input_0_pchx_pn.3410000000d8bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_input_0_pchx_pn.3410000000d8bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878228
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCG45980.2448052100171012No Hit
CCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGG43630.23229341698664913No Hit
TATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTG39480.21019812291159537No Hit
TGATGGCATTTGTAGGAGCCACCTTCCTTTTCCACTATCTTCACAATAAAG34770.18512129517822118No Hit
CGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATG30790.1639311095351576No Hit
CAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGATGCGGTG26320.1401320819410636No Hit
TAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTG26280.13991911525118356No Hit
GTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCG23780.12660869713368134No Hit
CTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACG23680.12607628040898122No Hit
CCTTCGAAGCCTGCTTTTTTGTACAAACTTGTGATATCACTAGTTTTTTGA23590.12559710535675117No Hit
AAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCG21900.11659926270931964No Hit
CCCCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCG21740.11574739594979949No Hit
TGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAG21570.11484228751780935No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC21310.11345800403358912TruSeq Adapter, Index 15 (98% over 51bp)
CTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG20640.10989081197809851No Hit
TAGTGTCACCTAAATAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTG20120.10712224500965804No Hit
CAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCGGATATTACCCTTTGT19250.10249021950476725No Hit
TCGCCTGGGACATCCTGTCCCCTCAGTTCCAGTACGGCTCCAAGGCCTACG18870.10046703595090692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCCC7600.040.55462628
GGACCCC7700.040.02794327
ACGTCTG9150.037.12695315
CCTGACG3100.037.01194810
TCCGCCC3050.036.881082
CCCCACC8450.036.74141331
ACCCCCA8400.036.6922829
TCCGCGC3200.036.5583712
GGTCCCA8550.036.04855715
TGGTCCC8500.035.99593414
TTCCGCG3250.035.9959311
CCCGCTT2950.035.84340737
TGGACCC8600.035.83897426
GCCCCCC3150.035.710255
CCCACCC8700.035.68562332
GCACACG9800.035.58271411
CCCCCAC8700.035.42703230
CGCGCAC3250.035.303714
CCCCCTG3250.035.30377
CTGCCGA8700.035.168444
Produced by FastQC (version 0.11.2)