FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_5_pchx_pn.3410000000d6f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_5_pchx_pn.3410000000d6f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462928
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGAC70071.5136263090588602No Hit
GTCCTGCAGGGAGGAGTCCTGGGTCACGGTCACCACGCCGCCGTCCTCGAA32110.6936283828154702No Hit
CAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCG26100.5638025783707186No Hit
CGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACA25740.5560259910828467No Hit
CTCTTCTCCATCAGCAGCTGAGAACACAACAGAGAAGCATAACAGTTTCCA21410.46249092731483077No Hit
TCCCTTGGACACACGGTCAAACATCAGCACAGCCACTAAACTCACCCAATG21290.45989873155220684No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC21250.45903466629799883TruSeq Adapter, Index 15 (98% over 51bp)
GCGCGTTTTGGCGACTCTCATTGCATATCTGTGTTGTTTGGTGATCAAGTA20170.4357049044343829No Hit
CACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGG20040.4328966923582068No Hit
TGATGACGTCCTCGGAGGAGGCCATCGGATCCTTATCGTCATCGTCGTACA19720.42598417032454294No Hit
CACCACGCAACCTCCGGTGGCGATGATGAGAAGATCAGCTCTGTCTCCGGT19250.41583140358759896No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC19130.4132392078249749TruSeq Adapter, Index 13 (98% over 51bp)
GAAAGCTGGGTCTCCACCTCCGGATCACAATTCTCCAGTGCTCTCGCAGGA18040.389693429647807No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA16570.3579390315556631No Hit
TATCGAACGTTCCTGCATCAACTTTCAGCCTCTTAGCCACTTCATCCTTCA13990.30220682265924725No Hit
CTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAA13990.30220682265924725No Hit
CTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCT13550.2927021048629593No Hit
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA13270.28665364808350324No Hit
TTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCA13260.28643763176995124No Hit
GGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTT13110.28319738706667125No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC12950.2797411260498393TruSeq Adapter, Index 14 (98% over 51bp)
GGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGA12450.2689403103722393No Hit
TATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTA12050.26029965783015935No Hit
GGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCTCCGTGAACGGCCACGA11980.2587875436352953No Hit
TCCCTTACTCTTCTCCATCAGCAGCTGAGAACACAACAGAGAAGCATAACA11840.2557633152455674No Hit
CACCTCATGCATGGAGTCCAGAAGCTTTGTCAGTTGGTAAAACCGTTGCCA11760.25403518473715136No Hit
GCATCCAACGCGTTGGGAGCTCTCCCATATGGGAGAGCTCAAGCTTCTGCA11330.24474648325441536No Hit
GTGCTGACAGTTGTATGTGAGACCCATAACTTGCCTCTCGCTCAGACTTGG11270.2434503853731034No Hit
CCCGGAGATCACCATGGACGACTTTCTCTATCTCTACGATCTAGGAAGAAA11030.2382659938478554No Hit
TCTCCTTACCTACTGCTTCCAGACATTTTTGGATAAGACCATGAGTATTGA10980.2371859122800954No Hit
GGAGTCCAGAAGCTTTGTCAGTTGGTAAAACCGTTGCCAGTTCTGACTGGA10070.21752842774686343No Hit
ATCTGCAGCTTCCTTCGCAATGCCGAACAGGCTTCTTGGTTCTATAGACCA9780.21126395465385547No Hit
GCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACT9350.20197525317111947No Hit
CGCTTCATTGTCGTCGGGCAAACTCCTAGGCTCTTTGCAGCGTCTTTGAGA9340.20175923685756747No Hit
TTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGA9320.2013272042304635No Hit
AGGGCCGCCACTCCACCGGCGCCTAAGAATTCGAGCTCGGTACCGGATCCA9290.20067915528980748No Hit
GCCACTGCAGGAGTCTCACAAGACACTTCGGAAAATCCTAACAAAACAATA9130.19722289427297549No Hit
GTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACG8980.1939826495696955No Hit
AACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCA8790.18987833961220751No Hit
AACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTA8740.1887982580444475No Hit
TGACCACTTGTTTTGCAATATCTCTCCAGAGCTCTTACACGGGCGACGACA8520.18404589914630354No Hit
TCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGA8450.18253378495143951No Hit
CATTGAGTCGTAAAAGACTCTGTATGAACTGTTCGCCAGTCTTCACGGCGA8280.17886150762105554No Hit
TGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAA8150.17605329554487956No Hit
GTCCAAGGGATGGAACTAACGGCCATGGAAGTCAGGTCGAGGCCTCCATCA8040.17367711609580758No Hit
CTCTCTACAAATCTATCTCTCTCTATTTTTCTCCAGAATAATGTGTGAGTA8030.17346109978225557No Hit
AATCTGTTCAAGGTACTGATGGAGGCCTCGACCTGACTTCCATGGCCGTTA7950.17173296927383955No Hit
AAACTTTGTCCACTTCGGGTGCATAGTGAATCTTCTCTGAGGTCATTATAA7900.17065288770607956No Hit
CGAGCTCGGTACCGGATCCAATTCCCGATCGTTCAAACATTTGGCAATAAA7480.1615802025368956No Hit
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAA7430.16050012096913557No Hit
TTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATA7380.15942003940137559No Hit
GGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCA7150.1544516641896796No Hit
GAATGTCTCGAGATCCCTAGATCCTCCCGCACGAAAATCGTAAGGCTCTTA7030.1518594684270556No Hit
GTATCCTATGAAGAGTATCTCTGTATGAAAACCTTACTGCTTCTCTCCTCA6850.1479711747831196No Hit
ATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAA6400.13825044067327963No Hit
TGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATA6100.13176995126671964No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC5160.11146441779283171TruSeq Adapter, Index 3 (97% over 37bp)
GATCTAGTGGCCATGAACCGTCAAGATCTAAGTCGTCAACATCCATTAGAA5110.11038433622507172No Hit
CTGCTGGAGGTGATTGAACCCGAGGTGTTGTATGCAGGATATGATAGCTCT5020.10844018940310371No Hit
AAGTTCAAAAGATCTGAGAAATCTCTGCCCCACTGCAGCATTTGACGATAA5010.1082241730895517No Hit
CCACGGTAGTATGAAGTGCTACTCGAAATCTTTGCCACCCGTCTCGTTCCA4930.10649604258113574No Hit
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA4780.10325579787785573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA2800.043.3876516
AGCGAAG2850.042.63106517
GATGGAC8400.042.04852710
GAGGCCC2900.041.905123
CACGAGG8500.041.82754524
CGTGGAA8400.041.780736
AGCATCG8350.041.77047731
CCACGAG8550.041.58743723
TGGACCC8550.041.5739612
GGACCCC8450.041.53347813
ACGTCTG8300.041.47092415
GGGTCAC3750.041.4084521
GGTCCCA8550.041.3555031
GCACACG8500.041.2891611
GGAGCAT8450.041.27615429
GCCCGCA3000.041.2539626
CGTATGC7750.041.22085644
GGCCCGC2950.041.19039525
GCACCGA2950.041.19039530
CGCACCG2950.041.19039529
Produced by FastQC (version 0.11.2)