FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_3hrs_pchx_pn.3410000000d836.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_3hrs_pchx_pn.3410000000d836.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253226
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCCGACCCATTTGCTGTCCACCAGTCATGCTAGAATCGATAGATCTGC40740.32508103087551643No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC38380.3062496309524379TruSeq Adapter, Index 3 (97% over 37bp)
CACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTG35880.28630111408477No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC25830.206108076276745TruSeq Adapter, Index 15 (98% over 51bp)
CAATCAAGTGGAAACCTGCTCTGCTTTGCTCCTGATCTGATTATTAATGAG23550.18791502889343184No Hit
CTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGG23220.18528182466689966No Hit
TGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCG22080.1761853009752431No Hit
CAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGG21410.1708390984547081No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC21240.16948259930770665TruSeq Adapter, Index 13 (98% over 51bp)
GATCTAAGTCGTCAACATCCATTAGAAGCTCCCTTGACCTCGAAGGTTGAG20830.16621104254140914No Hit
TTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGG20040.1599073112112261No Hit
AGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG19890.158710400199166No Hit
GTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGG19540.15591760783769248No Hit
CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGG18810.15009264091233346No Hit
CTCTGTATGAAAACCTTACTGCTTCTCTCCTCAGTTCCTAAGGAAGGTCTG18640.14873614176533204No Hit
CTCCTCTTCCTCTGAGCAGCCTTGCTCGCCTCTCTGGGCTTTCTCCGACGG18470.14737964261833061No Hit
CGAATCTCATCAAATAACTCTTGGCTCTTCAGACCTTCCTTAGGAACTGAG18350.14642211380868256No Hit
GTGGTGAACATGGTATCGTCACCTTCTCCGTCGAACTTTCTTCCTAGATCG17310.1381235307917327No Hit
AACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCG16830.13429341555314048No Hit
GTCTACAACCGACATGGCCTGCTATGTCGTGGATGCTCATGTCTGGGGCTT15310.12216471729759835No Hit
ATCGCCTTTGCCCATTTCACTGCTGCAATCACTTGACGCCCACCTAACATG15020.11985068934094888No Hit
CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGG14490.11562160376500329No Hit
GTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATT14390.11482366309029657No Hit
TCCTTGATGACGTCCTCGGAGGAGGCCATCGGATCCTTATCGTCATCGTCG14230.11354695801076581No Hit
CTCTAAAGCCCCAGACATGAGCATCCACGACATAGCAGGCCATGTCGGTTG14080.11235004699870574No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC13490.1076421970179361TruSeq Adapter, Index 18 (100% over 51bp)
GCCGCGTAGGCCTCTCTAACCATCTGTGGGTCAGCATTCTTTCTGAAATTG13170.10508878685887461No Hit
ATCCTGCATACAACACCTCGGGTTCAATCACCTCCAGCAGTGACACCAAGG13080.10437064025163856No Hit
TGCATTACGGGGCCGTCGGAGGGGAAGTTGGTGCCGCGCAGCTTCACCTTG12880.10277475890222514No Hit
TGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACG12540.10006176060822229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG15700.042.4169211
ACGTCTG15700.041.8437215
CGTCTGA16050.041.07961316
CACACGT16300.040.71753712
AGCACAC16550.040.64623310
ACACGTC16450.040.34625213
GAGCACA16850.039.5229
GTATGCC15450.038.89118645
GTCACGT7250.038.169329
CGTATGC15750.038.1488844
CTGAACT17050.038.14537419
CTCCAGT16950.037.9630224
TCTGAAC17250.037.83205818
TCTCGTA15950.037.66150741
AGATCGG38550.037.24471745
CTCGTAT16250.037.24460242
TCGGAAG17950.037.2253763
ACTCCAG17500.037.15707823
TCGTATG16350.037.15441543
AGTCACG8050.036.891428
Produced by FastQC (version 0.11.2)