FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_30_pchx_pn.3410000000d7e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_30_pchx_pn.3410000000d7e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385540
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCC29440.763604295274161No Hit
TCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACC28250.7327384966540437No Hit
CAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCC23160.6007158790268194No Hit
CGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCG23070.5983814908958862No Hit
CGTCGGAGGGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGC22990.5963064792239456No Hit
CGTTCACGGAGCCCTCCATGCGCACCTTGAAGCGCATGAACTCCTTGATGA22400.5810032681433832No Hit
CTTCAACGATGGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGAGCCAC22350.5797063858484204No Hit
CGTCACCTTCTCCGTCGAACTTTCTTCCTAGATCGTAGAGATAGAGAAAGT22030.5714063391606578No Hit
GCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGC20410.5293873528038595No Hit
TGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGTCACCACGCCGC20200.5239404471650153No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCGTCCATCTCGTATGC18540.4808839549722467TruSeq Adapter, Index 6 (97% over 36bp)
CCAAGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCC18210.47232453182549156No Hit
GGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGC17400.4513150386470923No Hit
GCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTC17280.44820252113918146No Hit
GGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGAGC16550.4292680396327229No Hit
CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTC16320.4233023810758936No Hit
CGGCGGCCACTACGACGCCGAGGTCAAGACCACCTACATGGCCAAGAAGCC16160.4191523577320122No Hit
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACCCGTCCATCTCGTATGC15700.40722104061835346RNA PCR Primer, Index 16 (100% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAAATCTCGTATGC14980.38854593557088757TruSeq Adapter, Index 13 (97% over 39bp)
ATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCC14600.3786896301291695No Hit
CGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTGGCCGC14580.3781708772111843No Hit
TGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGC14380.3729833480313327No Hit
CTTTCCTTTATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTAC14150.3670176894745033No Hit
AGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTC13750.35664263111480005No Hit
TGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCC13240.34341443170617836No Hit
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC13070.33900503190330444No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCATCTCGTATGC13010.33744877314934896TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACAACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC12340.32007055039684595TruSeq Adapter, Index 16 (97% over 36bp)
CGACGTCGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGC12170.3156611505939721No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC11830.3068423509882243TruSeq Adapter, Index 16 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCCGTCCATCTCGTATGC11460.2972454220054988Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC11420.29620791616952846TruSeq Adapter, Index 15 (98% over 51bp)
TCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACGGGGC11370.2949110338745655No Hit
CCTGAGCTACCAAACACCAATAATTCACCTAACCATTGGTCAAGTGATCAC10720.2780515640400477No Hit
GATCGGAAGAGCAACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC10600.27493904653213674TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAAGTCACCCGTCCATCTCGTATGC10190.2643046117134409Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC9970.2585983296156041TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAATCTCGTATGC9730.25237329459978214TruSeq Adapter, Index 16 (97% over 39bp)
TGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCC9540.24744514187892305No Hit
GAGGCCATCGGATCCTTATCGTCATCGTCGTACAGATCCCGACCCATTTGC9520.2469263889609379No Hit
GTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCC9110.23629195414224205No Hit
TCTTCTCATCATCGCCACCGGAGGTTGCGTGGTGAAAACCATTTCCTCCAC8980.2329200601753385No Hit
CTCCATCAGCAGCTGAGAACACAACAGAGAAGCATAACAGTTTCCAGTTTC8920.231363801421383No Hit
CCAGAAGCTTTGTCAGTTGGTAAAACCGTTGCCAGTTCTGACTGGAGTTCC8820.22877003683145716No Hit
TGTTCAAGGTACTGATGGAGGCCTCGACCTGACTTCCATGGCCGTTAGTTC8510.22072936660268716No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGGTCCATCTCGTATGC8500.22046999014369456TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACGTCCGCATCTCGTATGC8420.2183949784717539TruSeq Adapter, Index 18 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACATGTCAATCTCGTATGC8390.21761684909477616RNA PCR Primer, Index 15 (100% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC8380.21735747263578356TruSeq Adapter, Index 13 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACAGTTCCATCTCGTATGC8340.21631996679981327RNA PCR Primer, Index 14 (100% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAAATCTCGTATGC8280.21476370804585776TruSeq Adapter, Index 15 (97% over 39bp)
ACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCC8240.21372620220988744No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGGTCACCCGTCCATCTCGTATGC8130.21087306116096904Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTTACCGTCACATGAGCCATGAGAGGTTGGAGTCCCAGCGCTCTCATCCAC8120.21061368470197644No Hit
GATCGGAAGGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC8110.21035430824298387TruSeq Adapter, Index 16 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACCCGTCCATCTCGTATGC7950.20620428489910256TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACAACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC7860.20386989676816933TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC7790.20205426155522127TruSeq Adapter, Index 14 (98% over 51bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC7770.2015355086372361TruSeq Adapter, Index 15 (97% over 35bp)
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC7740.20075737926025833No Hit
GATCGGAAGAAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC7710.1999792498832806TruSeq Adapter, Index 16 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC7620.19764486175234738TruSeq Adapter, Index 18 (100% over 51bp)
TCCGGTGGCGATGATGAGAAGATCAGCTCTGTCTCCGGTGTTCCTTCTTTC7420.19245733257249573No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACATGTCAATCTCGTATGC7390.19167920319551796Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACAGTTCCATCTCGTATGC7160.1857135446386886Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACGTCCGCATCTCGTATGC7060.18311978004876278Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACAACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC7010.18182289775379987TruSeq Adapter, Index 18 (100% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAAGTCACGTCCGCATCTCGTATGC6990.1813041448358147Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACAACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC6900.17896975670488147TruSeq Adapter, Index 14 (97% over 36bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC6710.1740416039840224TruSeq Adapter, Index 18 (97% over 46bp)
GATCGGAAGAGCACACGTCTGGAACTCCAGTCACCCGTCCATCTCGTATGC6660.1727447216890595RNA PCR Primer, Index 16 (96% over 32bp)
GATCGGAAGAGCACACCGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC6640.17222596877107435RNA PCR Primer, Index 16 (96% over 31bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC6470.16781656896820046TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACGGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC6460.16755719250920786TruSeq Adapter, Index 16 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACAGTCAAATCTCGTATGC6400.16600093375525238Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCCTCGTATGC6250.16211028687036366TruSeq Adapter, Index 16 (97% over 42bp)
AAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTC6200.16081340457540075No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACGTCCGCATCTCGTATGC6180.16029465165741558TruSeq Adapter, Index 22 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACAGTCAAATCTCGTATGC6130.15899776936245266RNA PCR Primer, Index 13 (100% over 29bp)
GATCGGAAGAGCACCACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC6060.1571821341495046RNA PCR Primer, Index 16 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTTCGTATGC6050.156922757690512TruSeq Adapter, Index 18 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCCGCATCTCGTATGC6040.15666338123151943TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCCGCATCTCGTATGC6040.15666338123151943TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCAACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC5960.15458836955957878TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACCTCCAGTCACCCGTCCATCTCGTATGC5940.15406961664159363RNA PCR Primer, Index 16 (96% over 29bp)
GATCGGAAGAGCCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC5900.1530321108056233RNA PCR Primer, Index 16 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTCAATCTCGTATGC5780.1499195932977123TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAAGTCACATGTCAATCTCGTATGC5760.14940084037972715Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCACCCGTCCATCTCGTATGC5630.14602894641282357Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC5620.14576956995383097TruSeq Adapter, Index 15 (97% over 48bp)
GATCGGAAGAAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC5560.14421331119987552TruSeq Adapter, Index 18 (97% over 42bp)
GATCGGAAGAGCAACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC5550.14395393474088292TruSeq Adapter, Index 18 (100% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCCATCTCGTATGC5480.14213829952793483TruSeq Adapter, Index 14 (97% over 39bp)
GATCGGAAGAGCACACGTCCTGAACTCCAGTCACCCGTCCATCTCGTATGC5340.13850702910203871TruSeq Adapter, Index 16 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAAGTCACAGTTCCATCTCGTATGC5170.1340976292991648Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGAGACTCCAATCTCTATTACTTGCCTTGGTTTATGAAGCAAGCCTTGAAT5110.1325413705452093No Hit
GATCGGAAGAGCACAACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC5070.13150386470923897TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC5060.1312444882502464TruSeq Adapter, Index 18 (100% over 42bp)
GACGATTTCAACCCCTGGTCGACAAATCCTCGGACCCGGTCGACGATTCCT5020.13020698241427608No Hit
ACGTCCTCGGAGGAGGCCATCGGATCCTTATCGTCATCGTCGTACAGATCC5000.1296882294962909No Hit
GATCGGAAGAGCACACGGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC4980.12916947657830574TruSeq Adapter, Index 18 (100% over 34bp)
GATCGGAAGAGGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC4970.12891010011931317TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTTCCATCTCGTATGC4900.12709446490636508TruSeq Adapter, Index 14 (97% over 39bp)
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC4860.12605695907039477No Hit
GATCGGAAGGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC4710.12216631218550603TruSeq Adapter, Index 15 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCCGCATCTCGTATGC4680.12138818280852831TruSeq Adapter, Index 22 (97% over 39bp)
GAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGC4650.12061005343155055No Hit
ACCCCTGGTCGACCATTCCTCGAACCCGATAGATTTTCATCGACCATTCAT4610.11957254759558023No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACAGTCAAATCTCGTATGC4480.11620065362867667TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTTCGTATGC4460.1156819007106915TruSeq Adapter, Index 16 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGTCACGTCCGCATCTCGTATGC4430.11490377133371375Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGTCAATCTCGTATGC4350.11282875966177308TruSeq Adapter, Index 20 (97% over 37bp)
CGCCTAGGTTGGATGGGCGTGCTTCGTTGGAAAGCATGGATCCGCCTAGGC4230.10971624215386211No Hit
CTTTGATATAAGTCATTCGAATCTCATCAAATAACTCTTGGCTCTTCAGAC4230.10971624215386211No Hit
GATCGGAAGAGCACCACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC4210.10919748923587695RNA PCR Primer, Index 15 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGTCACAGTTCCATCTCGTATGC4210.10919748923587695Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACC4190.10867873631789178No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCCTCGTATGC4160.10790060694091405TruSeq Adapter, Index 18 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGTCAATCTCGTATGC4090.10608497172796597TruSeq Adapter, Index 12 (97% over 38bp)
GTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGC4080.1058255952689734No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTCCATCTCGTATGC4080.1058255952689734TruSeq Adapter, Index 14 (97% over 37bp)
ACTACAACGTGCGTGGTACACTGGCCTTGCCTGTCTTTAATCCCTCTGGTC4040.10478808943300305No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTTCCATCTCGTATGC4020.1042693365150179TruSeq Adapter, Index 7 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTTCGTATGC3990.10349120713804016TruSeq Adapter, Index 15 (97% over 43bp)
GATCGGAAGAGCACACGTCCTGAACTCCAGTCACATGTCAATCTCGTATGC3910.10141619546609949TruSeq Adapter, Index 15 (96% over 32bp)
GATCGGAAGAGCACACCGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC3880.10063806608912175RNA PCR Primer, Index 14 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACA502.1827873E-1144.9981849
GTACGCT253.886893E-544.99818440
CTATTAC600.044.9981815
CGCACAA2950.044.23550423
ATGCGCA2750.044.1857718
CGTCTGG2600.044.1385616
GATCGGA96600.044.0370981
CCATGCG2800.043.39673216
ATCGGAA98050.043.3919262
ATTACGG1350.043.33158542
GCACAAC4700.043.0833711
TCGGAAG94700.042.9074553
GTGTAGG3200.042.8888938
GGGATGA3100.042.8264116
ACGCACA3050.042.7907122
GACGCAC3050.042.7907121
GCACAAT3050.042.7851624
TGCGCAC2850.042.63538719
CCCAGTC5300.042.45111526
ACTCCCA5300.042.45111523
Produced by FastQC (version 0.11.2)