FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_10_pchx_pn.3410000000d77a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_10_pchx_pn.3410000000d77a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268621
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC73050.5758220934384659TruSeq Adapter, Index 15 (98% over 51bp)
CCACTGTCGGCAGAGGCATCTTCAACGATGGCCTTTCCTTTATCGCAATGA54050.4260531711204528No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC46990.37040219261702273TruSeq Adapter, Index 13 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC33090.26083440207910796TruSeq Adapter, Index 14 (98% over 51bp)
GCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCA32560.25665663740392125No Hit
CATTTGGTCATCCAGGTGTAAGTTTCTGAAGCCTGGTATCGCCTTTGCCCA27220.21456368765770079No Hit
CAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATC26490.20880940801074555No Hit
GAAAATCTTCGTCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAA24920.19643376548236233No Hit
CCACTGTCGGCAGGGGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGA22170.17475668462054467No Hit
CTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGA20580.1622233905949846No Hit
CGGCGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTGGGAGGTGA19590.15441964148473025No Hit
GGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGA19330.15237017202143116No Hit
ATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGA18210.1435416881795272No Hit
CGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGG17780.1401521809902248No Hit
CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA16670.13140252289690932No Hit
CTTCCCTTTTGACGATGGCTTTTCCTAGCTCTTTGATATAAGTCATTCGAA16600.1308507426567903No Hit
CTAACAAAACAATAGTTCCTGCTGCATTACCACAGCTCACCCCTACCTTGG15710.12383525103242024No Hit
GTGGAAACCTGCTCTGCTTTGCTCCTGATCTGATTATTAATGAGCAGAGAA15640.12328347079230124No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC15400.12139165282617898TruSeq Adapter, Index 3 (97% over 37bp)
CGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCAAGTAGTCGGGGA15380.12123400132900211No Hit
CTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCA14930.11768684264252287No Hit
ATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGT14420.11366672946451303No Hit
TACACCTGGATGACCAAATGACCCTGCTACAGTACTCATGGATGTTTCTCA14200.11193256299556764No Hit
CTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTA13120.10341938214801741No Hit
CAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATC13110.10334055639942899No Hit
CGCACATGGTTCTATCTCCTTCGAAGCCTGCTTTTTTGTACAAACTTGTGA12950.10207934442201415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25450.041.81494511
GTATGCC22650.041.62674745
CACACGT25800.041.42209612
ACACGTC25850.041.3419813
CGTCTGA26250.040.115216
AGCACAC26750.039.86692410
ACGTCTG26600.039.7534115
GAGCACA27800.038.9276739
CTGAACT27000.038.83421719
TCGGAAG28900.038.3802033
AGAGCAC28550.038.299088
GATCGGA31300.038.146521
ACTCCAG26950.037.90289323
GAACTCC27700.037.52940821
CCCGTCC1800.037.497933
CGTCCGC2100.037.497933
CGGAAGA29500.037.370794
TCCAGTC27150.037.292225
CGTATGC25450.037.22382444
CTCCAGT27400.037.19828824
Produced by FastQC (version 0.11.2)