FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 nlp7_chip_0_pchx_pn.3410000000d67b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 nlp7_chip_0_pchx_pn.3410000000d67b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759677
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC1082086.149310356389269TruSeq Adapter, Index 13 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC617793.5108147688467826TruSeq Adapter, Index 15 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC285461.6222295341701916TruSeq Adapter, Index 14 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC180861.0278022614377527TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC79960.4544015748344725TruSeq Adapter, Index 18 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC50310.28590474274540156TruSeq Adapter, Index 16 (98% over 51bp)
CACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCA35080.19935476794889062No Hit
TCCAAAACACTAACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGTA33980.19310362072130283No Hit
CTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGA26090.1482658465161504No Hit
TGGTTCCAAACCACCTGAGCTACCAAACACCAATAATTCACCTAACCATTG21380.12149957065984268No Hit
CTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCC21380.12149957065984268No Hit
CTGGAGTTCCCTTCCCTTTTGACGATGGCTTTTCCTAGCTCTTTGATATAA18870.10723558925871055No Hit
ACACTAACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGTAGCCAAA18790.10678096036943144No Hit
CGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCA17600.10001835564140465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC245850.044.7193145
CGTATGC246400.044.61949544
TCTCGTA246550.044.56902741
ATCTCGT247000.044.51515240
CTCGTAT247150.044.45956442
TCGTATG248250.044.3816443
GCACACG249250.044.32869311
ACGTCTG249850.044.26726515
CGTCTGA249900.044.2621816
CTGAACT249500.044.24547619
CACACGT250100.044.22301512
ACACGTC250350.044.2058113
CTCCAGT249450.044.1616324
ACTCCAG249500.044.1437623
AGCACAC250700.044.11717610
TCCAGTC250100.044.0558525
CAGTCAC250100.044.0443527
TCTGAAC250950.044.0142218
GAGCACA251450.043.9676939
ACAGTCA115100.043.9606732
Produced by FastQC (version 0.11.2)