FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 bzip1_chip_pchx_pn.3410000000d879.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 bzip1_chip_pchx_pn.3410000000d879.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418661
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTG43721.0442816503089611No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC21080.5035099997372575TruSeq Adapter, Index 15 (98% over 51bp)
GCATCTGTGCGGTATTTCACACCGCATCAGGTGGCACTTTTCGGGGAAATG20090.4798631828615515No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC18560.4433181022354602TruSeq Adapter, Index 13 (98% over 51bp)
AAGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTG17670.42205985272093655No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC16360.39076962028944656TruSeq Adapter, Index 18 (100% over 51bp)
CGACCCATTTGCTGTCCACCAGTCATGCTAGAATCGATAGATCTGCTAGAG15550.37142222466386887No Hit
GCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTCCTCGGGGTACA15280.3649730927886763No Hit
AAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTG13860.3310554362598857No Hit
GCAGATTTGACCCATGCAGCTACCGTCAAAGCTGGTGCAGTTTTTCTTTAG13500.32245659375962893No Hit
CGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCAAGTAGTCGGGGATG13280.31720174556502756No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC13230.31600746188443635TruSeq Adapter, Index 14 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC13020.3109914704259532TruSeq Adapter, Index 3 (97% over 37bp)
TTCACGGAGCCCTCCATGCGCACCTTGAAGCGCATGAACTCCTTGATGACG12770.30502005202299715No Hit
GTCTTTGTAACTTGCTTTGATTGTTACCGTTCTCATTTCTGAGCCTGATGG12360.2952269258421491No Hit
TGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGG12330.2945103556337944No Hit
CCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCG12070.28830008049472006No Hit
GCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGG12030.2873446535502471No Hit
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG11720.27994009473058157No Hit
TAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCT11630.2777903841055173No Hit
AACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCACGGTCACCACGCCGCCG11480.2742075330637437No Hit
CGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAG11200.26751954445243287No Hit
CCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAGCGCGCCGAGG10750.2567709913271119No Hit
TGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTG10400.24841100556297338No Hit
AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTG10220.24411158431284502No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC10120.2417230169516626TruSeq Adapter, Index 16 (98% over 51bp)
ATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGG9900.2364681687570612No Hit
CTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGTCGTAGTG9890.23622931202094297No Hit
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG9850.23527388507646996No Hit
ATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAG9500.22691389931233144No Hit
ATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGG9410.22476418868726727No Hit
TAGATCGTCTTGATGAGACCTGCCGCGTAGGCCTCTCTAACCATCTGTGGG9220.22022591070102066No Hit
GAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAG8920.21306020861747332No Hit
AGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACG8870.21186592493688208No Hit
GGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCG8290.19801223424202397No Hit
AGTTGTATGTGAGACCCATAACTTGCCTCTCGCTCAGACTTGGGTTCCATG8020.19156310236683138No Hit
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG6720.16051172667145974No Hit
TTGCTGTCCACCAGTCATGCTAGAATCGATAGATCTGCTAGAGTCAGCTTG5840.1394923338930543No Hit
GGCCATCGGATCCTTATCGTCATCGTCGTACAGATCCCGACCCATTTGCTG5710.13638719632351712No Hit
AGTATTTCCGAGCTTTTCAGATTTACCGCCTCAAGGGCTTTGCAAACTTTG4930.11775637090629411No Hit
TTCCCGCGTTTGCGTTCTGATCGTCGACACAATCGCCACCACCACTCTGCG4360.10414153694755424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG700.044.99725334
CACACGT11900.042.72848512
GCACACG12250.041.87499611
ACGTCTG12100.041.8362915
TCGGAAG12650.041.7958683
AGCACAC12450.041.5637310
ACACGTC12300.041.3389413
CGTCTGA12250.041.1452616
TGTGCGG2300.041.084456
CTAGCGC1150.041.084457
GAGCACA12800.040.778769
CGGAAGA13100.040.703624
GAGCCCT1400.040.1761177
ATCGGAA13250.040.0730252
TATGCCG3100.039.92168845
GATCGGA13550.039.883311
TCTGAAC12600.039.4665918
AGAGCAC13300.039.4148868
CCGCTTA1600.039.37259734
CGCTTAC1600.039.37259735
Produced by FastQC (version 0.11.2)