FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l01n01 bzip1_chip_input_pchx_pn.3410000000d9c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l01n01 bzip1_chip_input_pchx_pn.3410000000d9c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617085
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTGTAGGAGCCACCTTCCTTTTCCACTATCTTCACAATAAAGTGACAG36670.5942455253328147No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC32050.5193773953345163TruSeq Adapter, Index 13 (98% over 51bp)
GACTCCAAAACACTAACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGT31810.5154881418281112No Hit
TAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAG31060.5033342246205952No Hit
GCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCG30820.4994449711141901No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC28780.4663863163097466TruSeq Adapter, Index 15 (98% over 51bp)
CGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG25270.40950598377857184No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC23860.3866566194284418TruSeq Adapter, Index 14 (98% over 51bp)
CGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAG22330.3618626283251092No Hit
TATCCTGCATACAACACCTCGGGTTCAATCACCTCCAGCAGTGACACCAAG20000.3241044588670929No Hit
CCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAG19970.3236183021787922No Hit
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG18820.3049822957939344No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC18750.3038479301878996TruSeq Adapter, Index 18 (100% over 51bp)
CATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAG17620.2855360282619088No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC17410.28213293144380436TruSeq Adapter, Index 3 (97% over 37bp)
GATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCT17360.2813226702966366No Hit
CCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG17080.27678520787249733No Hit
CCTCGTTGTGGGAGGTGATGTCCAGCTTGATGTCGGTCTTGTAGGCGCCGG16450.26657591741818387No Hit
TCCATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAG15690.25425994798123436No Hit
GATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAG15220.24664349319785767No Hit
CCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGG14980.24275423969145254No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC14900.2414578218559842TruSeq Adapter, Index 16 (98% over 51bp)
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG14710.2383788294967468No Hit
CTCTCGCTCAGACTTGGGTTCCATGTCAGCATGGGAGCGTTCTTGCCAATG13260.21488125622888257No Hit
GTGAGACCCATAACTTGCCTCTCGCTCAGACTTGGGTTCCATGTCAGCATG13200.2139089428522813No Hit
CAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAG12740.20645454029833815No Hit
TGGTGTCACTGCTGGAGGTGATTGAACCCGAGGTGTTGTATGCAGGATATG12670.20532017469230332No Hit
TGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCG12620.20450991354513562No Hit
TCCCAGCGCTCTCATCCACCGTTCGTGATCGCTTGTGACCATTGCTTGGTG12480.20224118233306593No Hit
GCGTTCTTGCCAATGGTGGCGGTCTAAAGAAAAACTGCACCAGCTTTGACG12480.20224118233306593No Hit
CGATGATAATTCCGCTAGAAACGGCGTCACTACTCAACCTTCGAGGTCAAG12110.19624524984402475No Hit
CCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATG11490.18619801161914484No Hit
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG11470.18587390716027777No Hit
TTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCG10890.17647487785313207No Hit
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTG10670.17290972880559405No Hit
GCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG10650.17258562434672695No Hit
CCCGCACGAAAATCGTAAGGCTCTTAGTTCATGATGTAACGACAAATCTAG10200.16529327402221736No Hit
ACGAGCGCGCCGAGGGCCGCCACTCCACCGGCGCCTAAGAATTCGAGCTCG10060.1630245428101477No Hit
TCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAG9770.15832502815657487No Hit
TCTGGGCTTTCTCCGACGGTGGAGGAAATGGTTTTCACCACGCAACCTCCG9750.15800092369770777No Hit
CAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAG9590.15540808802677103No Hit
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT9260.150060364455464No Hit
GAACGGTGGATGAGAGCGCTGGGACTCCAACCTCTCATGGCTCATGTGACG8550.13855465616568222No Hit
GTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGGGGCATCTTGAACG7950.1288315223996694No Hit
TCTGAGCCTGATGGTTCGCATGCAACATTTGCAATGGCCTCTTCCTTTGGG6670.10808883703217548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA16850.042.59874316
AGCACAC17200.042.2483410
GCACACG17050.042.22417411
ACGTCTG17000.042.21602615
ACACGTC17100.042.10071613
TCGGAAG17600.042.0551153
CACACGT17150.041.97797412
AGAGCAC17400.041.892038
GAGCACA17450.041.6430669
CCGACTA3050.041.3070112
GCTCCGC3700.040.7388424
GTATGCC17000.040.6345545
TCTGAAC17800.040.45162618
GTCTGAA17700.040.42591517
CGTATGC17150.040.41034744
ATCTCGT17100.040.2588140
CGCTCAA3750.040.19565638
CTCGTAT17150.040.14468842
CTCGCTG3700.040.13730618
GTCCGCA2300.040.10436234
Produced by FastQC (version 0.11.2)