FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l08n01 sv84.3410000000c173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l08n01 sv84.3410000000c173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70865600
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC29669514.186729527443498TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3231650.044.486781
ATCGGAA3242600.044.345862
ACACGTC3220550.044.0727713
TCGGAAG3260050.044.0585253
CGTCTGA3218650.043.97316716
CGGAAGA3262950.043.9294854
ACGTCTG3226800.043.92062415
CACGTCT3247900.043.68155714
GCACACG3268550.043.51880611
TCTCGTA3202450.043.45857241
CACACGT3270250.043.44768512
CTCGTAT3204350.043.41983442
TCGTATG3219250.043.269943
CGTATGC3216850.043.24342344
CATCTCG3211350.043.2325139
ATCTCGT3224700.043.2086840
GTATGCC3244350.042.8795945
GATCATC3284900.042.30546636
GAACTCC3363050.041.9076321
AGTCACC3325950.041.90244328
Produced by FastQC (version 0.11.2)