FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l08n01 sv83.3410000000c116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l08n01 sv83.3410000000c116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26253406
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC10521474.007659044316003TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1151450.044.4462471
ATCGGAA1156300.044.287892
TCGGAAG1160000.044.103623
CGGAAGA1162150.043.9597434
ACACGTC1150150.043.95947313
ACGTCTG1150550.043.87192515
CGTCTGA1149800.043.86277816
CGCCAAT1130300.043.6487733
CACGTCT1161300.043.52190814
GTCACGC1138850.043.49930629
ACGCCAA1136500.043.48192632
TATCTCG1140100.043.4757839
GCACACG1165000.043.4615111
TCTCGTA1139800.043.42446541
CTCGTAT1141150.043.37401642
CACACGT1168800.043.30037712
AGTCACG1147150.043.2718528
TCACGCC1141900.043.2467430
ATCTCGT1145900.043.22635740
CACGCCA1143500.043.2039431