FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l08n01 sv82.3410000000c0ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l08n01 sv82.3410000000c0ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18451482
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1527920.8280744061642311TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG166350.041.9036639
CTCGTAT167500.041.72625442
GATCGGA174050.041.555231
CGTCTGA171200.041.55341716
TCTCGTA168100.041.50791541
TCGTATG169150.041.3990543
ACACGTC172700.041.3098613
CGTATGC170000.041.20528844
ACGTCTG173100.041.1886315
ATCGGAA177050.040.9808772
ATCTCGT172700.040.46735440
CGGAAGA179400.040.293354
TCGGAAG181300.039.9456443
CACGTCT180850.039.4357914
GCACACG183500.038.92779511
CACACGT184300.038.7219912
GTATGCC186150.037.65498745
AGTGATC192900.036.11287736
GTGATCT198600.035.14438237
GAACTCC206700.034.3187121
Produced by FastQC (version 0.11.2)