FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l08n01 sv81.3410000000c04e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l08n01 sv81.3410000000c04e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36665914
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC719620.19626402876524504TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA92200.035.36303341
CTCGTAT92450.035.26846342
AATCTCG94050.034.59494839
TCGTATG97700.033.4884443
CGTATGC100050.032.7018644
CGTCTGA106600.032.62816616
ATCTCGT102950.031.86675840
ACACGTC111250.031.5882513
GATCGGA115900.030.9878541
ATCGGAA117200.030.752472
ACGTCTG115250.030.39430415
CACGTCT124000.028.30397614
TCGGAAG134500.026.9806183
CGGAAGA139300.026.0023964
GTATGCC133050.025.03094545
CACACGT145100.024.42067512
GCACACG148900.023.84303711
ACCAATC146250.022.57027436
GACCAAT147100.022.53221735
CCAATCT167200.019.87681637
Produced by FastQC (version 0.11.2)