FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l08n01 sv80.3410000000bfe2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l08n01 sv80.3410000000bfe2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52773182
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC576610.10926193535193689TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA80250.032.77697434
TATCTCG83950.031.33215939
CACCGAT84600.031.3311231
TCTCGTA84650.031.17991441
CTCGTAT86000.030.74430542
ACCGATG86000.030.74258632
CCGATGT88850.029.756533
TCGTATG88800.029.74955243
CGTATGC93600.028.17585444
ATCTCGT95650.027.75847240
CGTCTGA104250.027.06228416
TCACCGA100550.026.54016730
ACACGTC109400.026.0559213
GTCACCG105500.025.18846729
ACGTCTG113200.024.90305315
GATCGGA116750.024.7870941
ATCGGAA117250.024.7144262
CACGTCT130400.021.92881214
TCGGAAG145650.020.1423283
GTATGCC134800.019.96515845
Produced by FastQC (version 0.11.2)