FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l07n01 alp_n2.3410000000c265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l07n01 alp_n2.3410000000c265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21496367
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAT21000.015.64272737
CCGTATC20800.015.3604538
AATCCGA36650.013.75111324
GTTCCGT23900.013.65038835
GATCGGA23050.013.579021
ACACGTC24850.013.49055213
ATCCGAA37050.013.35974825
GACGTAT17350.013.09741717
GACTACG17750.013.05610935
AGTTCCG25350.013.04683534
TCGTATG24700.013.02657245
TATCTCG25000.012.87040241
ACGTATA17800.012.76630318
AAATCCG39750.012.11268523
CGTATAG19550.011.96879219
ACGTCTG29800.011.92918115
ATCGGAA27650.011.3117792
AGACGTA20900.011.30336616
CTCCGAT44900.010.9830351
ACTACGT20450.010.89222936
Produced by FastQC (version 0.11.2)