FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l07n01 alp_eb2.3410000000c0d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l07n01 alp_eb2.3410000000c0d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27017269
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT19250.013.32409217
GACTACG19400.013.22148635
ACTACGT20200.012.92058436
ACGTATA20500.012.401895518
CGTATAG20850.012.3016219
AGACGTA22500.012.09955916
AATCCGA39850.011.68706724
GTCGCGA3357.9853635E-1011.4268221
CCCGATT15700.011.1870671
CTCCGAT60200.011.1465651
CTCGCGT4500.011.0085851
ATCCGAA41600.010.7627525
CCCGTTT38150.010.6832981
AAATCCG42300.010.63783223
CGCGTAA2352.1850834E-510.5401331
ACGTCTA25650.010.52630339
CCCGGAT46150.010.4903051
CTACGTC26050.010.27822237
CGGGCTA12150.010.1931331
CTCGTTT57750.010.0208021
Produced by FastQC (version 0.11.2)