FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l07n01 al_n2.3410000000c1ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l07n01 al_n2.3410000000c1ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23887003
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA30800.021.4204371
ACACGTC35350.018.7122313
TCGTATG35550.018.48173345
ATCGGAA36350.018.261412
AATCTCG37750.017.6432241
ACGTCTG40000.016.98691715
CGTCTGA42300.015.95676616
CTCGTAT41850.015.91463344
CACACGT49550.014.25792612
CACGTCT50150.013.45916114
TCTCGTA52100.013.17232643
GCACACG55350.012.92671211
ATTACGG28700.011.759539
TTACGGC27150.011.51988340
CTATACG11550.011.493978542
AGTCAAC59500.011.41968234
ATCCGAA34750.011.13616925
ATCTCGT63000.011.07188342
AATCCGA35950.011.01478324
AAATCCG35350.010.62892223
Produced by FastQC (version 0.11.2)