FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l07n01 al_eb2.3410000000c067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l07n01 al_eb2.3410000000c067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24734315
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA30200.020.0568921
ATCGGAA32400.018.6122532
TCGTATG35200.017.57884843
ACACGTC37250.016.4290113
AATCTCG38250.016.35288639
ACGTCTG39450.015.91204515
CGTATGC43400.014.20562644
CTCGTAT43500.014.172996542
CGTCTGA43900.013.99147116
GTATGCC46200.013.29589445
TCTCGTA47500.012.93213741
CACGTCT49600.012.70121114
GACCAAT50700.012.29273435
CACACGT53100.011.69454512
CGGAAGA58500.011.3075864
CTCCGAT48600.010.9807091
CTCGTTT46850.010.8621851
ACCAATC59400.010.71955336
GCACACG60150.010.51097411
ATCTCGT62000.010.27060440
Produced by FastQC (version 0.11.2)