FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l07n01 a_n2.3410000000c15a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l07n01 a_n2.3410000000c15a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28659213
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC20550.015.76577932
ATCCGAA65250.015.03378925
AATCCGA68700.014.24606724
TATCATG68550.014.2121176
AAATCCG67600.014.11177423
CGTCCCG23050.013.86113535
ACGTATA28700.013.79727318
GACGTAT29150.013.50709417
AGACGTA31400.012.75423216
GACTACG32400.012.43052435
GATCGGA26300.012.3290611
GCGGCTT79950.011.81952615
TTACGGC43550.011.67675440
ATTACGG46650.011.38270639
ATTAGGT65550.011.3614476
ACTACGT35300.011.3455836
ATCATGC94200.011.27354057
CCGCGTA7400.011.24949928
CGTATAG37650.011.05526619
TCCGAAG89500.010.98554526
Produced by FastQC (version 0.11.2)