FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l07n01 a_eb2.3410000000bfff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l07n01 a_eb2.3410000000bfff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21626850
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGA23550.016.43245730
TATCTCG23800.016.35509939
GATCGGA25050.015.1915661
ATCGGAA25450.014.8540432
TCGTATG27650.014.8105943
ACACGTC28000.014.06199613
ACGTCTG31500.013.0709615
GTCACCG33300.012.0265429
CGATGTA33350.011.73865634
CTCGTAT35250.011.68121542
TCTCGTA35750.011.20317341
CGTATGC36600.010.88148744
CGCGTGT5400.010.8418441
CGTCTGA38150.010.79248616
CACCGAT37100.010.67342331
GTATGCC38550.010.5644645
CACGTCT38400.010.37072314
GTCCTAT28550.010.2532321
CGCTAGT5201.8189894E-129.9597411
CACACGT40800.09.92618412
Produced by FastQC (version 0.11.2)