FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l06n01 bgm9.3410000000c130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l06n01 bgm9.3410000000c130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46005768
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC7730961.680432766604396TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA857250.044.1689951
ATCGGAA861900.043.8766942
TCGGAAG872850.043.2754973
ACACGTC866600.043.17957713
CGTCTGA864950.043.1246516
TCTCGTA854450.042.81265341
ACGTCTG872800.042.7857615
CGGAAGA881700.042.7747274
CTCGTAT855100.042.75642442
TCGTATG863350.042.39998243
CGTATGC863500.042.3900144
ATCTCGT864500.042.3483740
CACGTCT885800.042.21062514
CATCTCG864550.042.2031539
GCACACG894600.041.91726311
CACACGT898800.041.71784212
GTATGCC897150.040.85273745
GATCATC914550.039.9893736
GAACTCC958650.038.76641521
AGTCACC950900.038.6915928
Produced by FastQC (version 0.11.2)