FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l06n01 bgm8.3410000000c0b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l06n01 bgm8.3410000000c0b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49701253
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC5861641.1793746930283628TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA654200.043.581561
ATCGGAA661300.043.178412
CGCCAAT639900.042.90978633
CGTCTGA656600.042.73606516
ACACGTC658350.042.72101213
CTCGTAT647400.042.52065742
TCTCGTA647500.042.5140941
ACGCCAA647700.042.44167332
TCGGAAG672250.042.427133
GTCACGC650950.042.4050729
ACGTCTG664000.042.3241715
TATCTCG653500.042.19836839
CGTATGC655250.042.066244
TCGTATG655450.042.05336443
CGGAAGA677500.041.981924
AGTCACG662100.041.73168628
ATCTCGT660700.041.7153840
TCACGCC659700.041.69260830
CACGCCA659400.041.66380731
CACGTCT678300.041.481114