FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l06n01 bgm7.3410000000c031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l06n01 bgm7.3410000000c031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54727834
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC3998960.7306994828262343TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA465700.043.089581
ATCGGAA471100.042.6633382
CGTCTGA469000.041.58654416
ACACGTC471550.041.4755613
TCTCGTA459800.041.2680941
TCGGAAG486800.041.2396933
CTCGTAT461300.041.1924442
ACGTCTG475450.041.06970215
AATCTCG464750.040.88082539
TCGTATG467750.040.67252743
CGGAAGA493300.040.618544
CGTATGC469400.040.46723644
ATCTCGT475200.039.9678940
CACGTCT495350.039.4918814
GCACACG505600.038.95497511
CACACGT509350.038.5610712
GTATGCC514300.037.1093445
ACCAATC539950.035.0833136
GACCAAT551100.034.43065635
ACTGACC566750.033.70810332
Produced by FastQC (version 0.11.2)