FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l06n01 bgm6.3410000000bfd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l06n01 bgm6.3410000000bfd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52494545
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC13190152.512670602250196TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1438500.043.56162616
ACACGTC1442650.043.54802313
ATCGGAA1465850.043.5067372
GATCGGA1468150.043.468641
ACGTCTG1447800.043.3175515
CGATGTA1425650.043.24796334
ACCGATG1431050.043.2184132
CACCGAT1431050.043.21270431
TCGGAAG1474000.043.20363
TCTCGTA1426000.043.10355841
CCGATGT1432900.043.07467733
TATCTCG1430300.043.070739
CGGAAGA1474750.043.0518764
CTCGTAT1428150.043.0355242
CGTATGC1433300.042.9452644
TCGTATG1433950.042.93834343
GTCACCG1442450.042.90081429
CACGTCT1465500.042.8383214
ATCTCGT1437200.042.8345840
TCACCGA1448150.042.67136830