FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 sea157.3410000000c3c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 sea157.3410000000c3c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13350309
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC233320.17476749040040945TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAT48500.024.49491935
TAGCATC49900.023.8978736
GGTAGCA52200.022.9303334
CTCGTAT52400.022.80243542
GTATGCC55950.021.47515945
TCGTATG57400.021.20817643
AGTCACG59200.020.90338728
CGTATGC58750.020.64408544
ACGGTAG61550.019.8859632
CAGTCAC63100.019.6820727
TCTCGTA60900.019.65677841
CCAGTCA66000.019.08996626
CGGTAGC63050.018.9843533
CACACGT70350.018.03769112
AGGCTAG10500.018.0014743
ACACTAA18150.017.35425424
ACGTCTG73850.017.24394815
CGTCTGA73350.017.1774516
CTGAACT73950.017.06844119
CACGTCT75000.016.97897114
Produced by FastQC (version 0.11.2)