FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 sea156.3410000000c358.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 sea156.3410000000c358.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13846386
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC395690.28577131967865116TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT66500.030.18296442
ACGAGTG67150.030.12166832
CACGAGT68350.029.55991431
TCGTATG71250.028.23392943
TCACGAG73750.027.63988330
AGTCACG74250.027.30263928
GTATGCC73550.027.22687345
TCTCGTA74900.026.79805441
CAGTCAC77600.026.23948527
GTCACGA77650.026.16511229
CGTATGC77250.025.86613744
CGAGTGG79800.025.2900833
AGTGGAT82050.024.37843335
GAGTGGA87800.022.88324434
CCAGTCA91100.022.44987726
GGATCTC92550.021.73608438
ATCTCGT102050.019.88896640
TCCAGTC105450.019.6293825
TGGATCT103400.019.3668937
GTGGATC106500.019.09859336
Produced by FastQC (version 0.11.2)