FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 sea155.3410000000c2f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 sea155.3410000000c2f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16705876
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC1718021.0283926446000198TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA214500.039.50229334
CTCGTAT217850.038.97051642
GTACGAT221200.038.53071635
ACCGTAC222950.038.4498432
CCGTACG225100.038.0219433
TCGTATG227750.037.53339843
TCTCGTA227400.037.34401341
CACCGTA230500.037.2585331
AGTCACC231150.037.0763328
TACGATC230750.036.75079336
GTATGCC234550.036.35683445
CGTATGC239950.035.55919644
CAGTCAC244750.035.31863827
CCAGTCA264000.032.87115526
ATCTCGT264700.032.04751640
ACGATCT267800.031.84334637
TCACCGT272700.031.66617630
TCCAGTC274350.031.65539425
CTCCAGT279100.031.14083324
AACTCCA280800.031.03969822
Produced by FastQC (version 0.11.2)