FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 se401.3410000000c562.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 se401.3410000000c562.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10589068
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC757280.7151526461063429TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT93950.038.8484342
TCGTATG96750.037.86367843
TCACGTG99050.037.27574530
TCTCGTA99000.036.82131241
AGTCACG104300.035.52921728
CGTATGC106650.034.3907844
GTCACGT107650.034.33996629
GTATGCC107850.033.9014145
CAGTCAC111100.033.39432527
ATCTCGT112600.032.4732840
CCATCTC112300.032.46045738
CACGTGG116250.031.85715931
CCAGTCA116800.031.8416926
GCCATCT119550.030.48976337
CTCCAGT124300.030.01036324
CATCTCG124650.029.49653439
TCCAGTC129050.028.88832525
AACTCCA129400.028.82716822
ACTCCAG129750.028.73233623
ACGTGGC133700.027.6824632
Produced by FastQC (version 0.11.2)