FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 se400.3410000000c549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 se400.3410000000c549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15110008
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG836880.5538580786985685TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG118800.037.23419628
GTCACGT121100.036.50844229
CACGTGA124500.035.51095631
CTCGTAT123400.035.19249744
TCACGTG126350.035.04454430
TCGTATG126150.034.94120445
CAGTCAC128150.034.42906627
ACGATCT126550.033.48071339
AACGATC128050.033.0710538
CCAGTCA133350.032.9346526
TCCAGTC134650.032.68340725
CGATCTC130000.032.5577840
TCTCGTA134200.032.3104443
GATCTCG134350.032.1066741
CACGTCT139900.032.03544214
ACTCCAG137250.032.01498423
ACACGTC140200.031.98293713
ACGTGAA140300.031.6401532
GAACTCC139150.031.56146221
ACGTCTG142000.031.530315
Produced by FastQC (version 0.11.2)