FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 se399.3410000000c52f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 se399.3410000000c52f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18358604
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG462300.25181653245529995TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG82150.031.30478332
CTCGTAT82950.031.08728644
AGTCACG83600.030.89680328
TCGTATG86050.030.14891845
GTCACGT87000.029.45658529
GTCCGCA86600.029.22802734
CGCACAT88600.028.74781437
TCCGCAC88100.028.52732835
TCACGTC90150.028.50196330
CCGCACA91350.027.73407436
CGTCCGC93950.027.3006533
CAGTCAC95550.027.0554927
TCTCGTA96150.027.00689543
CACGTCC96250.026.6720831
CACATCT98750.025.88517639
ACATCTC102700.025.1308740
CCAGTCA102500.025.08929626
GCACATC105300.024.3179738
ATCTCGT109650.023.7639242
CATCTCG112500.023.06182941
Produced by FastQC (version 0.11.2)