FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 se398.3410000000c4f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 se398.3410000000c4f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28756257
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC1359460.4727527647287336TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAT183450.036.1691935
CTCGTAT184450.036.09859542
GTAGAGA184350.036.0893734
ACGTAGA187550.035.59382232
CGTAGAG193350.034.56077233
TCGTATG196000.034.1665343
TCTCGTA195850.033.9857141
TCACGTA197650.033.94571730
AGATCTC206500.032.11318238
AGTCACG210300.032.03247528
GAGATCT211450.031.39121637
GTCACGT217000.031.02271729
CACGTAG218500.030.84036831
GTATGCC222350.030.29672245
CGTATGC222650.030.14699444
CAGTCAC227300.029.63549427
ATCTCGT233550.028.4514140
CCAGTCA244150.027.6547126
AGAGATC248300.027.06700936
CTCCAGT263600.025.76767724
Produced by FastQC (version 0.11.2)