FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 se397.3410000000c4b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 se397.3410000000c4b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19818038
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG769120.3880908897237961TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT110250.036.22713544
GTCCCGA109550.035.67593436
TCGTATG115700.034.42186445
TCTCGTA118600.033.73391743
AGTCACC130100.031.68275528
ACCCGTC130300.031.65123432
CAGTCAC136750.030.22348627
CGTCCCG134200.030.17921435
CCGTCCC134950.030.0433234
CACCCGT141350.029.44750431
CCCGTCC138350.029.38630733
TCCCGAT136850.028.8061337
ATCTCGT140200.028.15148242
CCAGTCA147050.028.09120426
CTCCAGT163050.025.38944424
GTCACCC164900.025.35127429
TCCAGTC166900.024.81724725
ACTCCAG170850.024.32243223
AACTCCA171600.024.29454622
CCCGATC170250.023.3148938
Produced by FastQC (version 0.11.2)