FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 se396.3410000000c470.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 se396.3410000000c470.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20935219
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG4852142.31769249703096TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG595400.042.13289345
CTCGTAT596350.041.9328544
CACATGT602150.041.89793831
ACATGTC603450.041.7630432
TGTCAGA603600.041.54107735
TCACATG607900.041.49053230
AGTCACA607400.041.4807328
TCTCGTA603750.041.46833443
CATGTCA607450.041.42757833
ATGTCAG607900.041.3302934
AGAATCT607700.041.3056839
GAATCTC609900.041.18630240
GTCACAT612300.041.16347529
GTCAGAA615050.040.72017736
AATCTCG617950.040.66462741
CAGAATC618550.040.36343838
CAGTCAC623450.040.2778827
ATCTCGT626650.040.0283442
TCAGAAT626350.040.00852637
CCAGTCA638600.039.12856326
Produced by FastQC (version 0.11.2)