FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 se395.3410000000c420.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 se395.3410000000c420.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19066939
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG1248880.6549976375337436TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTC154550.039.74735340
CGTATCT155450.039.34342239
TCCGTAT159700.038.5202237
CTCGTAT162350.037.671244
TCGTATG164500.037.39612645
TCACAGT165200.037.2499230
TTCCGTA165100.037.17796736
TATCTCG166050.037.048941
CCGTATC166800.036.68015338
AGTCACA168800.036.6690528
GTCACAG168800.036.52242729
TCTCGTA168400.036.41181643
CACAGTT171600.035.99176831
GTTCCGT172250.035.4387935
ACAGTTC186150.033.16646232
ATCTCGT188450.032.7527442
CAGTCAC190150.032.6688127
AGTTCCG195350.031.46578434
CCAGTCA202200.030.72193126
TCCAGTC221500.028.0852225
Produced by FastQC (version 0.11.2)