FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 mk262.3410000000c2a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 mk262.3410000000c2a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5820977
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTT19400.024.8370821
CTCAGGC7750.022.6611231
CTACTAG13500.022.16914243
CCTGAGG14150.021.3074216
AGTCACC11450.021.22216228
CCCTAGT1301.2205419E-920.783871
ACTAGAT14450.020.70987745
CGGGCTT12850.020.6760441
GTCACCT12300.020.30435429
CAGGCTA5450.020.23161742
CACACGT12600.019.82040612
AGGCTAG5550.019.46163243
ACCTATA4300.018.83591513
CTGAGGG16100.018.72654717
CGCTAGT2550.018.5424731
GCACACG13400.018.46919811
AGGAACC16600.018.16155834
CAGCTAT17100.018.1568039
GACTAAC7850.018.056552
GCTACTA16700.018.05590842
Produced by FastQC (version 0.11.2)