FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 mk261.3410000000c215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 mk261.3410000000c215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26815110
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTT55400.023.6137331
GCCCTAG2400.021.5781841
CCCTAGT5250.020.1575091
CTACTAG46550.017.691943
CCTGAGG46100.017.66731516
GCCGGCT46850.017.5420991
CTGAGGG50500.016.21702817
CGCTATG26450.016.07755729
CCCGGGT16850.015.500881
CGGGCTT55150.015.4327951
CAGCTAT55000.015.2584029
ACTAGAT54350.015.23464145
ACCTATA16550.015.08958613
CTCAGGC30050.015.06086351
CACCTAT23200.015.03170412
AGCTATC55700.014.82433910
GCTATGG28600.014.79009230
GCCTAGT6100.014.7648291
CCTCTTA56000.014.46368611
GCCCGTT53350.014.3074931
Produced by FastQC (version 0.11.2)