FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l05n01 mk260.3410000000c1aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l05n01 mk260.3410000000c1aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40689845
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGT598260.14702931407086953No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGAA466740.11470675299942776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTT99450.027.2817541
CCTGAGG81400.019.95616516
GCCGGCT78550.019.4058421
CGCTATG39550.019.28587729
CCCTAGT6950.019.1142461
CTACTAG89200.018.28939843
CTGAGGG92350.017.711717
TAGCTTA18100.017.65147634
CCCCCTG10850.017.62815544
GCTATGG44250.017.49139430
CTCAGGC56800.017.3626381
ACCTATA47300.017.17101713
ACTAGAT95300.017.14098545
GCCCTAG2950.016.7915461
CGCGGCT61700.016.6771181
CACCTAT60900.016.51384212
GCCCGGT23850.016.3323141
CAGCTAT101600.016.3203959
CCTCTTA153700.016.27756311
CCGGTTT229000.016.0757881
Produced by FastQC (version 0.11.2)