FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk259.3410000000c413.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk259.3410000000c413.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25575888
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1799010.7034007968755571TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT269050.036.82788512
AGTCACG270100.036.1708128
CACGTCT275900.035.9377814
ACACGTC281000.035.36559313
CAGTCAC277700.035.1643527
GTCACGA276800.035.15730329
GCACACG283600.035.0900611
CAGATCT274350.035.00193837
CTCGTAT282300.034.49537742
AGATCTC284100.034.14956738
TCTCGTA286150.034.0627141
ACGTCTG293000.033.9555715
TCGTATG293650.033.3996143
AGCACAC300900.033.26700610
CGTCTGA301000.033.0157216
GTATGCC296800.032.8020345
GATCTCG299550.032.53093739
GTCTGAA306450.032.4064717
GATCAGA298050.032.3260234
TCTGAAC306400.032.30161318
Produced by FastQC (version 0.11.2)